Department of Experimental Neuroendocrinology

Scientific profile

  • About department
  • Employees
  • Laboratories

TECHNIQUES USED

Behavioural research:

Animal models: models of schizophrenia-like behaviours based on maternal immune activation using the administration of lipopolysaccharide or polyinosinic:polycytidylic acid to pregnant female rats, model of depressive-like behaviours based on the prenatal stress procedure, genetic depression model – Wistar Kyoto rats, model of co-occurrence of hypothyroidism and depression, model of Alzheimer’s disease-related characteristics, employing APPNL-F/NL-F knock-in mice, rat diabetes type I model (streptozotocin)

Behavioural tests: forced swim test (FST), prepulse inhibition test (PPI), water maze test, light-dark box test, social interactions test, exploratory and locomotor activities tests, novel object recognition test (NOR), Y-maze test, open field test (OFT), sucrose preference test

Methods of cellular biology:

Biochemical assays: cell viability/toxicity, enzymes activities, immunocytochemistry, live-cell imaging, flow cytometry, gene silencing and overexpression

Cell cultures in vitro and ex-vivo: primary neuronal, neuronal-glia, astrocyte and microglia cell cultures, cell lines (SH-SY5Y, U87-MG, C6, HT-22, HaCat, JAWSII), organotypic hippocampal and cortical cell cultures, immune cells tissue cultures

Cellular models: ischemia (OGD), excitotoxicity, oxidative stress, Parkinson’s disease, apoptosis, neuroiflammation, contact allergy

Molecular techniques:

Molecular methods to evaluate the levels of proteins or RNA in basic conditions or after exposure to drugs or other stimulants: qRT-PCR, ELISA, Western blot, Luminex

THE MOST IMPORTANT RECENT DISCOVERIES

  1. It has been shown that disturbances in the levels of neuronal and microglial proteins CD200-CD200R and CX3CL1-CX3CR1,as well as the accompamying changes in microglial reactivity, caused by the maternal immune activation during pregnancy, constitute an important element of the complex basis of the development of schizophrenia-like disorders in adulthood.
  2. In hippocampal organotypic cultures, in the ex vivo model of ischemia, dysfunction in the network of chemokines and their receptors, inflammatory activation and disturbed mechanotransduction mechanism, and consequently changes in the rigidity of organotypic cultures have been demonstrated.

FORMER EMPLOYEES OF THE DEPARTMENT:

Professor Bogusława Budziszewska, PhD

Lucylla Jaworska-Feil, PhD

Katarzyna Kotarska, PhD

Magdalena Szuster-Głuszczak, PhD

Magdalena Procner, PhD

Rugmani Meenambal, PhD

Danuta Jantas,PhD

Agnieszka Basta-Kaim

Professor Agnieszka Basta-Kaim, dr hab.

Head

+48 12 6623273
room 49

Pracownicy zakładu

Professor Marta Kubera, dr hab.

+48 12 6623263
room 49

Professor Władysław Lasoń, dr hab.

+48 12 6623378
room 127

Katarzyna Głombik, dr hab.

+48 12 6623394
room 120

Ewa Trojan, dr

+48 12 6623307
room 124

Katarzyna Curzytek-Malicka, dr

+48 12 6623394
room 120

Beata Grygier, dr

+48 12 6623394
room 120

Kinga Kamińska, dr

+48 12 6623258
room 126

Magdalena Kukla-Bartoszek, dr

+48 12 6623394
room 120

Monika Leśkiewicz, dr

+48 12 6623392
room 123

Krzysztof Łukowicz, dr

+48 12 6623250
room 52B

Magdalena Regulska, dr

+48 12 6623392
room 123

Kinga Tylek, dr

+48 12 6623258
room 126

Barbara Korzeniak

+48 12 6623392
room 123

Jakub Frydrych, mgr

+48 786829292

Natalia Bryniarska-Kubiak, dr

+48 12 6623258
room 126

Pracownicy pracowni

Professor Agnieszka Basta-Kaim, dr hab.

+48 12 6623273
room 49

Laboratory of Immunoendocrinology

Headdr hab. Agnieszka Basta-Kaim

Achievements

  • Publications
  • Grants
  • Awards

Grant

OPUS 22. The role of the immune checkpoints in the pathomechanism of depressive disorders

Professor Marta Kubera, PhD

Grant

OPUS 22. New approach to the treatment of Alzheimer's disease targeting resolution of inflammation and modulation of biomechanical properties of microglia by FPR2 hybrid agonists

Professor Agnieszka Basta - Kaim, PhD

Grant

GRIEG 1. Theranostic nanocarriers for drug delivery in central nervous system disorders

Professor Władysław Lasoń, PhD

Grant

CANALETTO 2021. Promoting resolution of inflammation as an innovate therapeutic strategy for central nervous system disorders characterized by neuroinflammation

Professor Agnieszka Basta - Kaim, PhD

Grant

SONATA 16. Prenatalne narażenie na glikokortykoidy jako czynnik ryzyka depresji – znaczenie mechanizmów epigenetycznych w metabolizmie mózgu w kontekście terapii.

Katarzyna Głombik, PhD

Grant

OPUS 20. New generation teranostatic nanocarriers for detection, diagnosis and neuroprotective treatment of ischemic stroke brain damage

Professor Władysław Lasoń, PhD

Grant

PRELUDIUM 18: Evaluation of molecular mechanisms of neuroprotective action of dental pulp stromal cells in an ex vivo organotypic model of ischemia

Natalia Bryniarska-Kubiak, PhD

Grant

Grant Joint Programme – Neurodegenerative Disease Research (JPND)

Professor Agnieszka Basta - Kaim, PhD

Grant

The project of Alzheimer's Association: Drug Development of Pro-resolving ALX/FPR2 Agonists for Alzheimer’s Disease

Professor Agnieszka Basta - Kaim, PhD

Grant

HARMONIA 9. Modulation of inflammatory processes using new agonists of the formyl peptide receptors ALX/FPR2 as a new therapeutic strategy of depression

Professor Agnieszka Basta - Kaim, PhD

Grant

OPUS 13: Efekty działania hormonów tarczycy w mózgu w zwierzęcym modelu depresji, w trakcie realizacji

Katarzyna Głombik, PhD

Grant

OPUS 10. The role of the neuron-microglia CX3CL1-CX3CR1 and CD200-CD200R protein systems in molecular mechanisms of antipsychotic drug actions: in vivo and in vitro study in the neurodevelopmental models of schizophrenia

Professor Agnieszka Basta - Kaim, PhD

Grant

PRELUDIUM 9: The role of the inflammasome NLRP3 in the mechanisms of antidepressant drugs action - studies in the animal model of depression

Ewa Trojan, PhD

Grant

PRLEUDIUM 7: Molecular mechanism of action of antidepressant drugs in the in vitro model of contact allergy in the human cell line HaCat and mouse dendritic precursor JAWSII cells

Katarzyna Curzytek - Malicka, PhD

Grant

PRELUDIUM 7: The impact of maternal diabetes on inflammasome NLRP3 activation in the offspring brain

Katarzyna Głombik, PhD

Award

Team Scientific Award of the Division of Medical Sciences of the Polish Academy of Sciences for a series of publications entitled "The importance of dysfunction of selected neuronal and microglial proteins in the prenatal period in the pathomechanisms of schizophrenia – studies in neurodevelopmental models"

Professor Agnieszka Basta - Kaim, PhD

Award

3rd place in the competition for a popular science article for young scientists of the Maj Institute of Pharmacology PAS: "Feel the force around you" - how do physical interactions affect cells?

Natalia Bryniarska-Kubiak, PhD

Award

The "Scientist of the Future 2021" award in the category: Science for a better life in the future

Natalia Bryniarska-Kubiak, PhD

Award

Scholarship of the President of the Polish Academy of Sciences

Natalia Bryniarska-Kubiak, PhD

Award

Scholarship for the best PhD students at the Maj Institute of Pharmacology, Polish Academy of Sciences (2020/2021)

Natalia Bryniarska-Kubiak, PhD

Award

Laureate of the competition organized by "Pharmacological reports" and director of May Institute of Pharmacology Polish Academy of Sciences for the best draft of review article

Natalia Bryniarska-Kubiak, PhD

Award

Scholarship of the Minister of Science and Higher Education for outstanding young scientists

Katarzyna Głombik, PhD

Award

The best work in the Pharmacy and Pharmacology session during the 10th National Conference on Advence in Biomedical Research in Warsaw

Natalia Bryniarska-Kubiak, PhD

Award

The main prize of Professor Kazimierz Ostrowski for the best work presented during the 10th National Conference on Advences in Biomedical Research in Warsaw

Natalia Bryniarska-Kubiak, PhD

Award

Neuron of The Audience: Best Poster on 9th Annual Conference Aspects of Neuroscience in Warsaw

Natalia Bryniarska-Kubiak, PhD

Award

Scholarship for the best PhD students at the Maj Institute of Pharmacology Polish Academy of Sciences (2019/2020)

Natalia Bryniarska-Kubiak, PhD

Award

3rd place in the competition for a popular science article for young scientists of the Polish Academy of Sciences

Natalia Bryniarska-Kubiak, PhD

Award

Stypendium wyjazdowe finansowane w ramach projektu Narodowej Agencji Wymiany Akademickiej (NAWA) PROM na udział w konferencji XIV European Meeting on Glial Cells in Health and Disease, Porto, Portugalia, 10-13.07.2019

Ewa Trojan, PhD

Award

Stypendium wyjazdowe na FENS Regional Meeting 2019 w ramach programu PROM Narodowej Agencji Wymiany Akademickiej

Katarzyna Głombik, PhD

Award

1st place for the best presentation of the E-poster: "The use of mesenchymal stem cells in the regeneration of nervous tissue after stroke - OGD model" during the 9th National Conference - Advances in Biomedical Research , Natalia Bryniarska-Kubiak

Natalia Bryniarska-Kubiak, PhD

In vivo effects of pentoxifylline on enzyme and non-enzyme antioxidant levels in rat liver after carrageenan-induced paw inflammation

Vircheva, S., Alexandrova, A., Georgieva, A., Mateeva, P., Zamfirova, R., Kubera, M., Kirkova, M.

DOI: 10.1002/cbf.1705

Increased 8-hydroxy-deoxyguanosine, a marker of oxidative damage to DNA, in major depression and myalgic encephalomyelitis / chronic fatigue syndrome

Maes, M., Mihaylova, I., Kubera, M., Uytterhoeven, M., Vrydags, N., Bosmans, E.

DOI:

Lower plasma Coenzyme Q10 in depression: A marker for treatment resistance and chronic fatigue in depression and a risk factor to cardiovascular disorder in that illness

Maes, M., Mihaylova, I., Kubera, M., Uytterhoeven, M., Vrydags, N., Bosmans, E.

DOI:

Inhibitory effects of amantadine on the production of pro-inflammatory cytokines by stimulated in vitro human blood

Kubera, M., Maes, M., Budziszewska, B., Basta-Kaim, A., Leśkiewicz, M., Grygier, B., Rogóz, Z., Lasoń, W.

DOI: 10.1016/S1734-1140(09)70173-2

Coenzyme Q10 deficiency in myalgic encephalomyelitis / chronic fatigue syndrome (ME/CFS) is related to fatigue, autonomic and neurocognitive symptoms and is another risk factor explaining the early mortality in ME/CFS due to cardiovascular disorder

Maes, M., Mihaylova, I., Kubera, M., Uytterhoeven, M., Vrydags, N., Bosmans, E.

DOI:

Prenatal stress decreases glycogen synthase kinase-3 phosphorylation in the rat frontal cortex

Szymańska, M., Suska, A., Budziszewska, B., Jaworska-Feil, L., Basta-Kaim, A., Leśkiewicz, M., Kubera, M., Gergont, A., Kroczka, S., Kaciński, M., Lasoń, W.

DOI: 10.1016/S1734-1140(09)70113-6

The inflammatory & neurodegenerative (I&ND) hypothesis of depression: Leads for future research and new drug developments in depression

Maes, M., Yirmyia, R., Noraberg, J., Brene, S., Hibbeln, J., Perini, G., Kubera, M., Bob, P., Lerer, B., Maj, M.

DOI: 10.1007/s11011-008-9118-1

Concomitant administration of fluoxetine and amantadine modulates the activity of peritoneal macrophages of rats subjected to a forced swimming test

Roman, A., Rogóz, Z., Kubera, M., Nawrat, D., Nalepa, I.

DOI: 10.1016/S1734-1140(09)70169-0

Study of the cytotoxicity and antioxidant capacity of N/OFQ(1-13)NH<inf>2</inf>and its structural analogues

Kirkova, M., Zamfirova, R., Leśkiewicz, M., Kubera, M., Lasoń, W., Todorov, S.

DOI: 10.1016/S1734-1140(09)70179-3

The effect of antidepressant drugs on the HPA axis activity, glucocorticoid receptor level and FKBP51 concentration in prenatally stressed rats

Szymańska, M., Budziszewska, B., Jaworska-Feil, L., Basta-Kaim, A., Kubera, M., Leśkiewicz, M., Regulska, M., Lasoń, W.

DOI: 10.1016/j.psyneuen.2008.12.012

Head

Professor Agnieszka Basta-Kaim, dr hab.

+48 12 6623273
room 49

See also