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Department of Experimental Neuroendocrinology
Scientific profile
- About department
- Employees
- Laboratories
TECHNIQUES USED
Behavioural research:
Animal models: models of schizophrenia-like behaviours based on maternal immune activation using the administration of lipopolysaccharide or polyinosinic:polycytidylic acid to pregnant female rats, model of depressive-like behaviours based on the prenatal stress procedure, genetic depression model – Wistar Kyoto rats, model of co-occurrence of hypothyroidism and depression, model of Alzheimer’s disease-related characteristics, employing APPNL-F/NL-F knock-in mice, rat diabetes type I model (streptozotocin)
Behavioural tests: forced swim test (FST), prepulse inhibition test (PPI), water maze test, light-dark box test, social interactions test, exploratory and locomotor activities tests, novel object recognition test (NOR), Y-maze test, open field test (OFT), sucrose preference test
Methods of cellular biology:
Biochemical assays: cell viability/toxicity, enzymes activities, immunocytochemistry, live-cell imaging, flow cytometry, gene silencing and overexpression
Cell cultures in vitro and ex-vivo: primary neuronal, neuronal-glia, astrocyte and microglia cell cultures, cell lines (SH-SY5Y, U87-MG, C6, HT-22, HaCat, JAWSII), organotypic hippocampal and cortical cell cultures, immune cells tissue cultures
Cellular models: ischemia (OGD), excitotoxicity, oxidative stress, Parkinson’s disease, apoptosis, neuroiflammation, contact allergy
Molecular techniques:
Molecular methods to evaluate the levels of proteins or RNA in basic conditions or after exposure to drugs or other stimulants: qRT-PCR, ELISA, Western blot, Luminex
THE MOST IMPORTANT RECENT DISCOVERIES
- It has been shown that disturbances in the levels of neuronal and microglial proteins CD200-CD200R and CX3CL1-CX3CR1,as well as the accompamying changes in microglial reactivity, caused by the maternal immune activation during pregnancy, constitute an important element of the complex basis of the development of schizophrenia-like disorders in adulthood.
- In hippocampal organotypic cultures, in the ex vivo model of ischemia, dysfunction in the network of chemokines and their receptors, inflammatory activation and disturbed mechanotransduction mechanism, and consequently changes in the rigidity of organotypic cultures have been demonstrated.
FORMER EMPLOYEES OF THE DEPARTMENT:
Professor Bogusława Budziszewska, PhD
Lucylla Jaworska-Feil, PhD
Katarzyna Kotarska, PhD
Magdalena Szuster-Głuszczak, PhD
Magdalena Procner, PhD
Rugmani Meenambal, PhD
Danuta Jantas,PhD
Pracownicy zakładu
Professor Marta Kubera, dr hab.
+48 12 6623263
kubera@if-pan.krakow.pl
room 49Professor Władysław Lasoń, dr hab.
+48 12 6623378lason@if-pan.krakow.plroom 127Katarzyna Głombik, dr hab.
+48 12 6623394glombik@if-pan.krakow.plroom 120Ewa Trojan, dr
+48 12 6623307trojan@if-pan.krakow.plroom 124Katarzyna Curzytek-Malicka, dr
+48 12 6623394curzytek@if-pan.krakow.plroom 120Beata Grygier, dr
+48 12 6623394grygier@if-pan.krakow.plroom 120Kinga Kamińska, dr
+48 12 6623258k.kamin@if-pan.krakow.plroom 126Magdalena Kukla-Bartoszek, dr
+48 12 6623394mkuklaba@if-pan.krakow.plroom 120Monika Leśkiewicz, dr
+48 12 6623392leskiew@if-pan.krakow.plroom 123Krzysztof Łukowicz, dr
+48 12 6623250lukowicz@if-pan.krakow.plroom 52BMagdalena Regulska, dr
+48 12 6623392regulska@if-pan.krakow.plroom 123Kinga Tylek, dr
+48 12 6623258tylek@if-pan.krakow.plroom 126Barbara Korzeniak
+48 12 6623392room 123Jakub Frydrych, mgr
+48 786829292frydrych@if-pan.krakow.plNatalia Bryniarska-Kubiak, dr
+48 12 6623258natbry@if-pan.krakow.plroom 126Pracownicy pracowni
Achievements
- Publications
- Grants
- Awards
Grant
OPUS 22. The role of the immune checkpoints in the pathomechanism of depressive disorders
Professor Marta Kubera, PhD
Grant
OPUS 22. New approach to the treatment of Alzheimer's disease targeting resolution of inflammation and modulation of biomechanical properties of microglia by FPR2 hybrid agonists
Professor Agnieszka Basta - Kaim, PhD
Grant
GRIEG 1. Theranostic nanocarriers for drug delivery in central nervous system disorders
Professor Władysław Lasoń, PhD
Grant
CANALETTO 2021. Promoting resolution of inflammation as an innovate therapeutic strategy for central nervous system disorders characterized by neuroinflammation
Professor Agnieszka Basta - Kaim, PhD
Grant
SONATA 16. Prenatalne narażenie na glikokortykoidy jako czynnik ryzyka depresji – znaczenie mechanizmów epigenetycznych w metabolizmie mózgu w kontekście terapii.
Katarzyna Głombik, PhD
Grant
OPUS 20. New generation teranostatic nanocarriers for detection, diagnosis and neuroprotective treatment of ischemic stroke brain damage
Professor Władysław Lasoń, PhD
Grant
PRELUDIUM 18: Evaluation of molecular mechanisms of neuroprotective action of dental pulp stromal cells in an ex vivo organotypic model of ischemia
Natalia Bryniarska-Kubiak, PhD
Grant
Grant Joint Programme – Neurodegenerative Disease Research (JPND)
Professor Agnieszka Basta - Kaim, PhD
Grant
The project of Alzheimer's Association: Drug Development of Pro-resolving ALX/FPR2 Agonists for Alzheimer’s Disease
Professor Agnieszka Basta - Kaim, PhD
Grant
HARMONIA 9. Modulation of inflammatory processes using new agonists of the formyl peptide receptors ALX/FPR2 as a new therapeutic strategy of depression
Professor Agnieszka Basta - Kaim, PhD
Grant
OPUS 13: Efekty działania hormonów tarczycy w mózgu w zwierzęcym modelu depresji, w trakcie realizacji
Katarzyna Głombik, PhD
Grant
OPUS 10. The role of the neuron-microglia CX3CL1-CX3CR1 and CD200-CD200R protein systems in molecular mechanisms of antipsychotic drug actions: in vivo and in vitro study in the neurodevelopmental models of schizophrenia
Professor Agnieszka Basta - Kaim, PhD
Grant
PRELUDIUM 9: The role of the inflammasome NLRP3 in the mechanisms of antidepressant drugs action - studies in the animal model of depression
Ewa Trojan, PhD
Grant
PRLEUDIUM 7: Molecular mechanism of action of antidepressant drugs in the in vitro model of contact allergy in the human cell line HaCat and mouse dendritic precursor JAWSII cells
Katarzyna Curzytek - Malicka, PhD
Grant
PRELUDIUM 7: The impact of maternal diabetes on inflammasome NLRP3 activation in the offspring brain
Katarzyna Głombik, PhD
Award
Team Scientific Award of the Division of Medical Sciences of the Polish Academy of Sciences for a series of publications entitled "The importance of dysfunction of selected neuronal and microglial proteins in the prenatal period in the pathomechanisms of schizophrenia – studies in neurodevelopmental models"
Professor Agnieszka Basta - Kaim, PhD
Award
3rd place in the competition for a popular science article for young scientists of the Maj Institute of Pharmacology PAS: "Feel the force around you" - how do physical interactions affect cells?
Natalia Bryniarska-Kubiak, PhD
Award
The "Scientist of the Future 2021" award in the category: Science for a better life in the future
Natalia Bryniarska-Kubiak, PhD
Award
Scholarship of the President of the Polish Academy of Sciences
Natalia Bryniarska-Kubiak, PhD
Award
Scholarship for the best PhD students at the Maj Institute of Pharmacology, Polish Academy of Sciences (2020/2021)
Natalia Bryniarska-Kubiak, PhD
Award
Laureate of the competition organized by "Pharmacological reports" and director of May Institute of Pharmacology Polish Academy of Sciences for the best draft of review article
Natalia Bryniarska-Kubiak, PhD
Award
Scholarship of the Minister of Science and Higher Education for outstanding young scientists
Katarzyna Głombik, PhD
Award
The best work in the Pharmacy and Pharmacology session during the 10th National Conference on Advence in Biomedical Research in Warsaw
Natalia Bryniarska-Kubiak, PhD
Award
The main prize of Professor Kazimierz Ostrowski for the best work presented during the 10th National Conference on Advences in Biomedical Research in Warsaw
Natalia Bryniarska-Kubiak, PhD
Award
Neuron of The Audience: Best Poster on 9th Annual Conference Aspects of Neuroscience in Warsaw
Natalia Bryniarska-Kubiak, PhD
Award
Scholarship for the best PhD students at the Maj Institute of Pharmacology Polish Academy of Sciences (2019/2020)
Natalia Bryniarska-Kubiak, PhD
Award
3rd place in the competition for a popular science article for young scientists of the Polish Academy of Sciences
Natalia Bryniarska-Kubiak, PhD
Award
Stypendium wyjazdowe finansowane w ramach projektu Narodowej Agencji Wymiany Akademickiej (NAWA) PROM na udział w konferencji XIV European Meeting on Glial Cells in Health and Disease, Porto, Portugalia, 10-13.07.2019
Ewa Trojan, PhD
Award
Stypendium wyjazdowe na FENS Regional Meeting 2019 w ramach programu PROM Narodowej Agencji Wymiany Akademickiej
Katarzyna Głombik, PhD
Award
1st place for the best presentation of the E-poster: "The use of mesenchymal stem cells in the regeneration of nervous tissue after stroke - OGD model" during the 9th National Conference - Advances in Biomedical Research , Natalia Bryniarska-Kubiak
Natalia Bryniarska-Kubiak, PhD
Lowered Quality of Life in Long COVID Is Predicted by Affective Symptoms, Chronic Fatigue Syndrome, Inflammation and Neuroimmunotoxic Pathways
Michael Maes, Haneen Tahseen Al-Rubaye, Abbas F. Almulla, Dhurgham Shihab Al-Hadrawi, Kristina Stoyanova, Marta Kubera, Hussein Kadhem Al-Hakeim
DOI: 10.3390/ijerph191610362
Adverse Childhood Experiences Predict the Phenome of Affective Disorders and These Effects Are Mediated by Staging, Neuroimmunotoxic and Growth Factor Profiles
Michael Maes, Muanpetch Rachayon, Ketsupar Jirakran, Pimpayao Sodsai, Siriwan Klinchanhom, Monojit Debnath, Agnieszka Basta-Kaim, Marta Kubera, Abbas F. Almulla, Atapol Sughondhabirom
DOI: 10.3390/cells11091564
The effects of pessimism on cell-mediated immunity in rats
Curzytek, K., Kubera, M., Trojan, E., Wójcik, K., Basta-Kaim, A., Detka, J., Maes, M., Rygula, R.
DOI: 10.1016/j.pnpbp.2017.04.034
Stimulatory effect of desipramine on lung metastases of adenocarcinoma MADB 106 in stress highly-sensitive and stress non-reactive rats
Grygier, B., Kubera, M., Wrona, D., Roman, A., Basta-Kaim, A., Gruca, P., Papp, M., Rogoz, Z., Leskiewicz, M., Budziszewska, B., Regulska, M., Korzeniak, B., Curzytek, K., Glombik, K., Slusarczyk, J., Maes, M., Lason, W.
DOI: 10.1016/j.pnpbp.2017.04.024
Deficit schizophrenia is a discrete diagnostic category defined by neuro-immune and neurocognitive features: results of supervised machine learning
Kanchanatawan, B., Sriswasdi, S., Thika, S., Sirivichayakul, S., Carvalho, A.F., Geffard, M., Kubera, M., Maes, M.
DOI: 10.1007/s11011-018-0208-4
Targeting the NLRP3 inflammasome-related pathways via tianeptine treatment-suppressed microglia polarization to the M1 phenotype in lipopolysaccharide-stimulated cultures
Ślusarczyk, J., Trojan, E., Głombik, K., Piotrowska, A., Budziszewska, B., Kubera, M., Popiołek-Barczyk, K., Lasoń, W., Mika, J., Basta-Kaim, A.
DOI: 10.3390/ijms19071965
In major affective disorders, early life trauma predict increased nitro-oxidative stress, lipid peroxidation and protein oxidation and recurrence of major affective disorders, suicidal behaviors and a lowered quality of life
Moraes, J.B., Maes, M., Roomruangwong, C., Bonifacio, K.L., Barbosa, D.S., Vargas, H.O., Anderson, G., Kubera, M., Carvalho, A.F., Nunes, S.O.V.
DOI: 10.1007/s11011-018-0209-3
Suppression of pro-inflammatory cytokine expression and lack of anti-depressant-like effect of fluoxetine in lipopolysaccharide-treated old female mice
Duda, W., Kubera, M., Kreiner, G., Curzytek, K., Detka, J., Głombik, K., Ślusarczyk, J., Basta-Kaim, A., Budziszewska, B., Lasoń, W., Regulska, M., Leśkiewicz, M., Roman, A., Zelek-Molik, A., Nalepa, I.
DOI: 10.1016/j.intimp.2017.04.021
The modulatory properties of chronic antidepressant drugs treatment on the brain chemokine - Chemokine receptor network: A molecular study in an animal model of depression
Trojan, E., Ślusarczyk, J., Chamera, K., Kotarska, K., Glombik, K., Kubera, M., Basta-Kaim, A.
DOI: 10.3389/fphar.2017.00779
Depression in cancer: The many biobehavioral pathways driving tumor progression
Bortolato, B., Hyphantis, T.N., Valpione, S., Perini, G., Maes, M., Morris, G., Kubera, M., Köhler, C.A., Fernandes, B.S., Stubbs, B., Pavlidis, N., Carvalho, A.F.
DOI: 10.1016/j.ctrv.2016.11.004